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Macrogen 454 life gs-flx instrument
454 Life Gs Flx Instrument, supplied by Macrogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/454+flx+instrument/454+gs+flx++platform/pm29052965-192-11-17
Average 90 stars, based on 1 article reviews
454 life gs-flx instrument - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

DNA Sequencing:

Article Title: Global Survey and Genome Exploration of Bacteriophages Infecting the Lactic Acid Bacterium Streptococcus thermophilus .
Article Snippet: PCR reactions were conducted in a total volume of 25 μl using Taq polymerase (Qiagen, Hilden, Germany). .. Reaction conditions were as follows: initial denaturation of 95◦C for 2min, 30 cycles of 95◦C for 15 s, 55◦C for 30 s, and 72◦C for 1 min, with a final extension of 72◦C for 10 min. DNA Sequencing and in Silico Analysis PhageDNA samples exhibiting unique restriction pattern profiles were sequenced using one of two methods, as follows: (i) random shotgun sequencing using pyrosequencing technology was performed (Macrogen, Inc., Geumcheon-gu, Seoul, South Korea) using a 454 FLX instrument yielding at least 65-fold coverage for each genome, with the exception of phage 7632, which yielded 17-fold coverage. .. Individual sequence files were assembled using GSassembler (454 Lifesciences, Branford, CT, U.S.A.), generating a consensus sequence for each phage; (ii) The remaining genomes were determined by GenProbio srl (University of Parma, Italy) using a MiSeq sequencer (Illumina, USA).

In Silico:

Article Title: Global Survey and Genome Exploration of Bacteriophages Infecting the Lactic Acid Bacterium Streptococcus thermophilus .
Article Snippet: PCR reactions were conducted in a total volume of 25 μl using Taq polymerase (Qiagen, Hilden, Germany). .. Reaction conditions were as follows: initial denaturation of 95◦C for 2min, 30 cycles of 95◦C for 15 s, 55◦C for 30 s, and 72◦C for 1 min, with a final extension of 72◦C for 10 min. DNA Sequencing and in Silico Analysis PhageDNA samples exhibiting unique restriction pattern profiles were sequenced using one of two methods, as follows: (i) random shotgun sequencing using pyrosequencing technology was performed (Macrogen, Inc., Geumcheon-gu, Seoul, South Korea) using a 454 FLX instrument yielding at least 65-fold coverage for each genome, with the exception of phage 7632, which yielded 17-fold coverage. .. Individual sequence files were assembled using GSassembler (454 Lifesciences, Branford, CT, U.S.A.), generating a consensus sequence for each phage; (ii) The remaining genomes were determined by GenProbio srl (University of Parma, Italy) using a MiSeq sequencer (Illumina, USA).

Shotgun Sequencing:

Article Title: Global Survey and Genome Exploration of Bacteriophages Infecting the Lactic Acid Bacterium Streptococcus thermophilus .
Article Snippet: PCR reactions were conducted in a total volume of 25 μl using Taq polymerase (Qiagen, Hilden, Germany). .. Reaction conditions were as follows: initial denaturation of 95◦C for 2min, 30 cycles of 95◦C for 15 s, 55◦C for 30 s, and 72◦C for 1 min, with a final extension of 72◦C for 10 min. DNA Sequencing and in Silico Analysis PhageDNA samples exhibiting unique restriction pattern profiles were sequenced using one of two methods, as follows: (i) random shotgun sequencing using pyrosequencing technology was performed (Macrogen, Inc., Geumcheon-gu, Seoul, South Korea) using a 454 FLX instrument yielding at least 65-fold coverage for each genome, with the exception of phage 7632, which yielded 17-fold coverage. .. Individual sequence files were assembled using GSassembler (454 Lifesciences, Branford, CT, U.S.A.), generating a consensus sequence for each phage; (ii) The remaining genomes were determined by GenProbio srl (University of Parma, Italy) using a MiSeq sequencer (Illumina, USA).

Article Title: Global Survey and Genome Exploration of Bacteriophages Infecting the Lactic Acid Bacterium Streptococcus thermophilus
Article Snippet: .. Phage DNA samples exhibiting unique restriction pattern profiles were sequenced using one of two methods, as follows: (i) random shotgun sequencing using pyrosequencing technology was performed (Macrogen, Inc., Geumcheon-gu, Seoul, South Korea) using a 454 FLX instrument yielding at least 65-fold coverage for each genome, with the exception of phage 7632, which yielded 17-fold coverage. .. Individual sequence files were assembled using GSassembler (454 Lifesciences, Branford, CT, U.S.A.), generating a consensus sequence for each phage; (ii) The remaining genomes were determined by GenProbio srl (University of Parma, Italy) using a MiSeq sequencer (Illumina, USA).



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Image Search Results


Comparison of the old and the new S. salmonicida genome assemblies.

Journal: Scientific Data

Article Title: A chromosome-scale reference genome for Spironucleus salmonicida

doi: 10.1038/s41597-022-01703-w

Figure Lengend Snippet: Comparison of the old and the new S. salmonicida genome assemblies.

Article Snippet: Sequencing instrument , 454 FLX (Illumina GA IIx*) , PacBio RS II.

Techniques: Comparison, Sequencing

Journal: Data in Brief

Article Title: Draft genome data of Prunus avium cv ‘Stella’

doi: 10.1016/j.dib.2022.108611

Figure Lengend Snippet:

Article Snippet: Single, 8 kb paired-end (PE), and 20 kb PE reads were generated using pyrosequencing on the 454 GS FLX instrument, and 100 bp PE reads were generated using Illumina 2000 instrument.

Techniques: Next-Generation Sequencing, Sequencing, Generated, Produced, Genome Wide